PRODUCTION OF BIOETHANOL FOR ENZYMATIC CONVERSION

Abstract

The lignocellulosic materials as agricultural waste (sugar cane bagasse, rice husks, and others) contain polymerized sugars, as cellulose and hemicellulose, which can be hydrolyzed by acid or enzyme, releasing glucose. This study aimed to obtain bioethanol from hydrolyzed sugar cane bagasse by enzyme and fermented using Saccharomyces cerevisiae. After washing, drying and grinding, the cake was delignified with sodium hydroxide (10% w/w dry base) under heat and pressure of 1.2 atm. The enzymatic hydrolysis of the delignified solution was carried out with commercial enzymes NS50013 (cellulase), NS50010 (β-glucosidase), NS50030 (xylanase), NS22002 (hemicellulase) and NS50012 (multicomponenti complex) obtained from Novozymes Latin América Ltd. After this process, the hydrolyzate was fermented with Saccharomyces cerevisiae, and distillate. The results showed that the yield of ethanol was of 16.42% (w/w). Key words: Ethanol. Lignocellulosic residue. Biofuels. Bioconversion. Lignolitic enzymes. Agroindustry residue.